Polymorphism of Bovine Lymphocyte Antigen DRB3.2 Alleles in Iranian Native Sarabi Cows
نویسندگان
چکیده
Sarabi cows (n = 136) from the Sarabi Breeding Station were genotyped at bovine lymphocyte antigen (BoLA)DRB3.2 locus by a genotyping system that used the polymerase chain reaction and restriction fragment length polymorphism. Genomic DNA was extracted from whole blood samples. A two-step polymerase chain reaction was carried out in order to amplify a 284 base-pair fragment of target gene. Nested-PCR products were digested with three restriction endonuclease enzymes RsaI, BstYI and HaeIII. Digested fragments were analyzed by polyacrylamide gel electrophoresis. Twenty-six BoLA-DRB3.2 alleles were identified with frequencies ranging from 0.4 to 15.1%. Six new allele types observed in this study have not been reported previously. Identified alleles include: BoLA-DRB3.2*1, *2, *4, *6, *8, *12, *13, *14, *15, *16, *17, *23, *24, *25, *28, *32, *34, *35, *36, *37, *42, *46, *51, *kba, *laa and *vaa. Their frequencies were found to be 0.4, 0.4, 0.7, 11.4, 1.1, 1.8, 2.9, 2.2, 4.4, 9.6, 1.1, 13.6, 0.4, 0.4, 1.1, 0.7, 0.4, 6.2, 2.2, 3.7, 1.1, 7.7, 1.5, 15.1, 2.6 and 7.3% respectively. The six most frequent alleles (DRB3.2 *6, *16, *23, *46, *kba and *vaa) accounted for 64.7% of the alleles in the population of this herd. Numerous studies on this locus, covering different breeds, has revealed the existence of various alleles in this locus, and new investigations have introduced novel alleles. With respect to the high number of the observed alleles in this survey and the novelty of some alleles with no previous record of reporting, it is plausible to conclude that the BoLA-DRB3.2 locus is highly polymorphic in Iranian native Sarabi cows. (Asian-Aust. J. Anim. Sci. 2006. Vol 19, No. 6 : 775-778)
منابع مشابه
Polymorphism of Bovine Lymphocyte Antigen DRB3.2 in Holstein Bulls of Iran Using PCR-RFLP
The Holstein bulls (n=50) were genotyped for bovine lymphocyte antigen (BoLA-DRB3.2) alleles by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Genomic DNA was extracted from bull semen using phenol-chloroform method. A two-step PCR was conducted in order to amplify a 284 base-pair fragment of the target gene. Amplicons were digested by RsaI, HaeIII and BstyI ...
متن کاملDetection of haplotype in the Bovine Prion Protein gene (PRNP) in Najdi, Sarabi and Iranian Holstein cattle breeds
ABSTRACT: Transmissible spongiform encephalopathies are neurodegenerative diseases that affect several mammalian species. Bovine spongiform encephalopathy (BSE) is among the mentioned diseases in cattle caused by abnormally fold prion proteins. Polymorphisms of the prion protein gene (PRNP) have been shown to be major determinations for the susceptibility to transmitted perion diseases in human...
متن کاملAnalysis of variations, structures, and phylogenic characteristics of bovine leukocyte antigen DRB3 exon2
Bovine leukocyte antigen (BoLA) DRB3 is a highly polymorphic gene in major histocompatibility complex(MHC) class II that plays a central role in immune responses and production factors. As of yet, molecular andevolutionary characteristics of BoLA-DRB3.2* have not been as fully understood as human and mouse.Therefore, we attempted to analyze variability and phylogeny of BoLA-DRB3.2* and illustra...
متن کاملAssociation of gene BoLA DRB3.2 with production traits in a dairy herd of Antioquia, Colombia Asociación del gen BoLA DRB3.2 con características productivas en un hato lechero de Antioquia, Colombia
Objective. To determine the associations of BoLA DRB3.2 alleles present in Holstein and BON x Holstein cattle to production and milk quality traits in a dairy herd of Antioquia, Colombia. Materials and methods. Ninety-one cows, 66 Holstein and 25 BxH, were genotyped for the BoLA DRB3.2 gene, through PCR-RFLP technique. Furthermore, the association of the alleles of the gene BoLA DRB3.2 with mil...
متن کامل